- The Bradford assay is based on the binding of the basic amino acids of a protein to a dye present in the Bradford reagent (e.g. Coomassie brilliant blue).
- dmjnThis leads to a shift in the absorbance maximum of the dye from 465 to 595.
- Bradford assay standard curve protocol depends on creating a standard curve of protein solutions with known concentrations, the protein concentration of unknown samples can be plotted on the standard dilution curve and calculated through Bradford assay analysis.