
The Amylase Test is a fundamental biochemistry laboratory experiment used to qualitatively detect salivary amylase and evaluate its ability to hydrolyze starch into simpler sugars. By observing iodine color changes, students can measure enzyme activity under different temperature and pH conditions to identify the optimum environment for starch digestion. This experiment also introduces essential laboratory techniques, proper handling of equipment, and the principles of enzyme catalysis. Through the Amylase Test, learners gain practical insights into how environmental factors influence enzyme function and develop a deeper understanding of biochemical reactions involved in carbohydrate metabolism.