The Urease Test is a widely used biochemical test in microbiology that determines whether microorganisms produce the enzyme urease, which hydrolyzes urea into ammonia and carbon dioxide. This reaction increases the pH of the medium, causing the phenol red indicator to change from its original color to deep pink, indicating a positive result. The test is especially valuable for distinguishing rapid urease-producing organisms such as Proteus species from other lactose non-fermenting enteric bacteria. Commonly used in microbiology laboratories and education, the Urease Test helps students understand bacterial metabolism, enzyme activity, and the identification of clinically important microorganisms.