PCR Virtual Lab is an interactive simulation designed to teach students how to amplify DNA fragments using the Polymerase Chain Reaction technique. Through this virtual experience, learners practice essential laboratory skills, understand the role of key reagents such as Taq polymerase and primers, and follow the core PCR steps: denaturation, annealing, and extension. The lab enables students to visualize how DNA is replicated in cycles to produce millions of copies from minimal samples. It also highlights the importance of PCR in microbiology for detecting and studying microorganisms, while preparing students for real laboratory applications in a controlled, risk-free environment.