The Real Time PCR Test is a powerful molecular biology technique used to amplify and quantify nucleic acids, including extracted DNA and cDNA synthesized from RNA. Unlike conventional PCR, it monitors DNA amplification in real time through fluorescent detection using SYBR Green dye. This test helps students understand the complete PCR workflow, including denaturation, primer annealing, and extension, while gaining practical experience with real-time PCR protocols. The technique is widely applied in gene expression analysis, viral load quantification, genomic profiling, and allelic discrimination. By performing a Real Time PCR Test, learners develop essential laboratory skills and data interpretation abilities.