Cloning-Growth and Plasmid Extraction by Alkaline Lysis Method

To apply the sterile technique while growing the bacterial culture.
To perform properly the steps of how to isolate plasmid DNA from bacteria using the plasmid extraction alkaline lysis method for plasmid isolation protocol.
To understand the role of the devices, the reagents, and the enzymes used in the plasmid extraction by alkaline lysis method.
To implement proper storage of samples to understand the purpose of plasmid isolation.